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Quantitative PCR (qPCR) is a cornerstone of molecular biology, used for applications ranging from gene expression analysis to pathogen detection. However, accurate qPCR results depend on precise liquid handling, making pipetting a critical yet time-consuming step.
Quantitative PCR (qPCR) is essential for gene expression analysis and pathogen identification in life science labs. While qPCR is sensitive, its plate preparation is time-consuming and requires skilled technicians. Automation enhances efficiency and reproducibility. This study compared manual pipettes, the semi-automated TRACKMAN® Connected, and the fully automated PIPETMAX® 268 liquid handler. All tools provided precise results with varying degrees of automation. Choose manual, semi-automated, or fully automated solutions to optimize processes, improve traceability, reduce errors, and free up time for critical tasks.
Oligonucleotides are core components of many molecular biology tools available today, including antisense gene therapy and primers for qPCR. Depending on the application, highly pure oligos are typically required, or experiments lack reproducibility — and for applications such as therapeutics, the consequences can be dire. To ensure that oligos are at the desired level of purity for their application, you need to use effective purification techniques. HPLC is one of the most effective and versatile approaches available for oligo purification, and in this article, we cover five tips to help improve your HPLC workflow to help you achieve highly pure oligos every time.
Synthetic oligonucleotides (oligos) are central to many cutting-edge biochemical applications across research and therapeutics. While solid-phase synthesis typically yields oligos with relatively high purity, they usually need further purification to avoid unintended side-reactions and interferences in downstream applications. Preparative HPLC is the preferred method for small to medium length oligos (10-60 bp). To increase productivity and maximize the profitability of your oligo research and development, finding the optimal LC purification platform is crucial.
Quantitative polymerase chain reaction (qPCR) is a powerful and highly sensitive method of amplifying genetic material, with applications ranging from diagnostics and forensics to research.
However, ensuring the accuracy and reliability of your qPCR experiments can be challenging — even the smallest amount of contamination or failure to adhere strictly to liquid handling best practices can substantially impact your results.
So, what can you do to maximize your chances of delivering highly accurate qPCR results, time after time? In this article, we share our top 5 tips for qPCR success.
When choosing a high performance liquid chromatography (HPLC) system, it's important to consider how your future needs may evolve from your current needs. In this article, you’ll learn about the advantages of modular HPLC systems when it comes to current experimental needs and how the ability to swap out HPLC components safeguards the future of your system and your research.
Switching from a manual to an automated process not only enables significantly more effective use of personnel time, but also removes operator error for reproducible results and increases the accuracy and precision of assays. In this article, we delve into the challenges that can arise during this transition and explain how Gilson's solutions and services can ease the process of transferring your manual methods to an automated system.
The vinegar fly, or fruit fly, is an excellent animal model for studying the underlying mechanisms of human disease. In preparation for use in research studies, fruit fly larvae must eat and grow continuously over a four-day period before hatching. In this app note, we describe the time-consuming and labor-intensive process of preparing vessels with fly food and how to avoid it using a fully automated system.
Did you know that about 75% of known human disease genes have a recognizable match in the genome of the fruit fly? In this technical note, a lab uses the Gilson Fly Food Dispenser to dispense fly food into vials at regular intervals.
The quantitative polymerase chain reaction (qPCR) has transformed the world of molecular biology methodology by enabling fast, high-throughput detection and quantification of DNA and RNA in real time. PIPETMAX 268 is specifically designed to maximize reproducibility and accuracy in qPCR workflows. Here, we explore three ways that PIPETMAX 268 can help you run more effective and efficient experiments.
